Metagenome Analysis of Protein Domain Collocation within Cellulase Genes of Goat Rumen Microbes
نویسندگان
چکیده
منابع مشابه
Metagenome Analysis of Protein Domain Collocation within Cellulase Genes of Goat Rumen Microbes
In this study, protein domains with cellulase activity in goat rumen microbes were investigated using metagenomic and bioinformatic analyses. After the complete genome of goat rumen microbes was obtained using a shotgun sequencing method, 217,892,109 pair reads were filtered, including only those with 70% identity, 100-bp matches, and thresholds below E(-10) using METAIDBA. These filtered conti...
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A carboxymethyl cellulase gene, cel5B, was cloned, sequenced, and expressed in Escherichia coli. pRCS20 in E. coli was identified from metagenomic cosmid library of cow rumen for cellulase activity on a carboxymethyl cellulose agar plates. Cosmid clone (RCS20) was partially digested with Sau3AI, ligated into BamHI site of pBluescript II SK+ vector, and transformed into E. coli DH5α. The insert ...
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OBJECTIVE This experiment was conducted to explore the impact of diet on the ruminal microbial community in goats. METHODS Twelve goats were divided into two groups and fed complete feed (CF) or all forage (AF) diet. The total microbial DNAs in the rumen liquid were extracted. The V4 region of microbial 16S rRNA genes was amplified and sequenced using high-throughput. Information of sequences...
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the current thesis is composed in five chapters in the following fashion: chapter two encompasses the applied framework of the project in details; the methodology of carl gustav jung to explain the process of individuation, the major archetypes and their attributes and his techniques to assess the mind’s strata are all explained. moreover, the austrian psychoanalysts, heinz kohut’s models of a...
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ژورنال
عنوان ژورنال: Asian-Australasian Journal of Animal Sciences
سال: 2013
ISSN: 1011-2367,1976-5517
DOI: 10.5713/ajas.2013.13219